Peptide shelf-life and freeze thaw method
07-24-2009 03:17 AM
Peptide shelf-life and freeze thaw method
As we all know, that once peptides are reconstituted they have a life of around 12-14 days before degrading, if not sooner. This means most people are wasting money, and peptide. I for one love my peptides and my money, and have found a method enables one to use small doses, and not worry about ruining a whole vial of a peptide.
After trail and error, and endless research found a very simple and effective method. Here are the steps
1- recon a peptide as you normally would.
2- draw out half of the newly reconed peptide into a slin pin (with 5mg peptides I usually add 2 cc's of ba water, and with 2mg peptides 1 cc of Ba water when I recon new peptides)
3-Be sure the pin is air free, seal in a plastic bag with no air in it.
4- place slin pin in freezer untill you use up the first half of the peptide.
5- when its time to thaw the new pin, just move it into the fridge, and within a few hours should be thawed.
6- inject the contents of the thawed pin back into the orig vial.
I wondered about this damaging the peptide but from countless times of doing it, and observing effects of peptides concluded little to no degrade occured. Flushing from the cjc was still very profound, and extreme hunger after doses over 200mcg of ghrp-6.
This ensures me that I am able to do low doses of the peptides over a longer span of time, without worring about my peptides degrading.
You can freeze more than one pin per vial, but i usually just take out half and freeze that...so far so good.
The LORD is my rock, my fortress, and my savior; my God is my rock, in whom I find protection. He is my shield, the power that saves me, and my place of safety.-Psalm 18:2
07-24-2009 11:00 AM
awesome man. thanks for sharing your work. that degradation is a biatch
07-27-2009 12:32 PM
Been doing a method similar to yours, definitely a good idea.
07-30-2009 01:11 AM
07-30-2009 01:18 AM
and a little more info on shelf life...
Originally Posted by datBtrue;
The reconstitution agent for both CJC-1295 & GHRP-6 is Bacteriostatic Water (BW).
Here are a few interesting snippets from studies on storage:
The following paragraph comes from a study that sent the peptide home with study participants for self-administration.
Vials were stored frozen until dispensed to the subjects, then kept at 4 C for 10 days at home. High performance liquid chromatographic analysis showed that the peptide was stable at 4 C for at least 2 weeks.
The following seems to indicate that GHRP-6 which is a simple peptide chain unlike IGF-1 which is more complex, is relatively resistant to degradation under the right circumstances at room temperature for almost five years.
The influence of the various buffer species (acetate, citrate, phosphate and borate) was shown to be different and the maximum stability of GHRP-6 was revealed to be in acetate buffer of pH 5.5-6.0. Degradation of GHRP-6 was greater in citrate-containing buffers than in acetate-containing ones. Furthermore, in the citrate-containing buffers, the higher buffer concentration caused greater degradation than the lower ones, but the concentration effect was negligible in acetate-containing buffers. Aqueous solution of GHRP-6 buffered with acetate (0.01 M, pH 5.5) showed a predicted t90% of 4.73 years at 20°C. - Degradation kinetics of growth hormone-releasing hexapeptide (GHRP-6) in aqueous solution, In Sik Ha… International Journal of Pharmaceutics Volume 144, Issue 1, 22 November 1996, Pages 91-97
5.5.4 Storage in solid state
Lyophilized powders can be quite stable as long as they are not reconstituted.
For example, Activase® lyophilized powder shows no significant loss of
bioactivity after storage for more than 4 years at controlled room temperature.
Degradation in the solid state often takes place by aggregation. The
stability of a protein in the solid state is dependent on the moisture content
of the solid, temperature, and composition of the formulation....
These degradation factors become more important the longer the chain of amino acids becomes & with the inclusion of amino acids sensitive to degradation.
Storage of unreconstituted peptides in frozen form (devoid of moisture) is always far more preferable for long-term storage then reconstituted peptides.
GHRP-6 is a simple chain with no extra-sensitive amino acids. But Growth Hormone Releasing Hormone (GHRH) is more sensitive because it is a longer chain composed of some sensitive amino acids. The added bioconjugation complex of CJC-1295 appears to be stable and shouldn't effect degradation/life of the peptide it is attached to...GHRH.
To answer stability questions the best way is to find studies that specifically examine the stability of the peptide you are interested in.
We have already looked to a very comprehensive study of GHRP-6 now lets look at a stability study for a slightly modified GHRH (and by undeclared assumption CJC-1295).
Investigation of the chemical stability of a new growth hormone-releasing hormone (GHRH) analogue by HPLC, M Idei, I Mezo, EZ Szabo, and G Keri, Biomed Chromatogr, March 1, 1996; 10(2): 89-91
The stability of a new active growth hormone-releasing hormone analogue (D-Ala2,Nle27,(gamma-amino-butyric acid)30-GHRH(1-30)-NH2) was investigated during storage at different temperatures in aqueous solution. Samples stored for various periods of time were analysed by HPLC.
It is concluded that in aqueous solution D-Ala2, Nle27,(gamma-amino-butyric acid)30-growth hormone-releasing hormone (1-30)-NH2 is stable: at least for 36 days at 4 degrees C; for 28 days at 25 degrees C; and for 10 days at 37 degrees C.
07-30-2009 06:06 PM
Originally Posted by TripDog
Cool I'll just sitback and let everyoneelse give the good info!!!
08-07-2012 03:08 AM
If we leave in room temp (28C) reconstuted vial for some hours, it is destroyed?
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