Beelzebub
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lion exposed yet again
for those of you that use lion products, here's another warning to stay away.
posted by liftsiron:
Lion's IGF-1 http://www.premiermuscle.com/forum/showthread.php?t=5957
It was tested by a ph.d chemist at an indepedant lab, here is what was found.
The test is conclusive. There is BSA in the IGF-1 and a buttload of it! The lighter signal for HSA may come from the cross-reactivity I mentioned (very likely).
We also tested it to see if it was
1. receptor grade
2. human IGF-1.
There was initially some ambiguity as to whether some of the IGF was porcine (pig) because of some slight cross reactivities. The diluted experiment clarified that. It is human and it is receptor grade. unfortunately, we ran out of sample before we could do the diluted run to determine if the HSA signal was just cross reactivity related or if there is actually a little HSA in there along with the gobs of BSA. I am 99% certain there is no HSA and here is why:
Pharmaceutical preps of IGF-1 do exist but they are the whole intact protein, not just the binding region (receptor grade). No receptor grade IGF or other receptor grade protein was ever developed for human use or even animal use. Receptor grade proteins are strictly used to determine binding constants and stuff like that in a petre dish. Since receptor grade was never intended for human use, it is produced in growth medium that contains BSA as a stabilizer (BSA is cheap). There would be no incentive to use more expensive HSA to stabilize something not intended for human use. When it was lyophylized (freeze dried), the BSA was included to stabilize the LR3 IGF-1. Without it, the IGF-1 would just fall apart in storage.
Lion does not make this stuff. There are only a handful of places that do make it (5 to be exact). He has to buy it from one of those companies. They all likely use BSA to stabilize their product.
This stuff isn't fit to inject into an animal, little lone a human being.
I will try to be more clear in my expanation here: Lion's stuff was applied to a plate with BSA antibodies in one column and HSA antibodies in another separate column. When Lion's stuff was applied to antibodies for BSA they lit up like Chernobyl! Definite positive for BSA. There was also a lighter reaction with the HSA antibodies. This was caused by our overloading the sample and causing a little cross reactivity. BSA and HSA are very similar proteins after all.
Just for argument's sake, let's say that it was HSA and no BSA was present. What we would have seen is the opposite. The HSA antibodies would have lit up brightly and the BSA antibodies would have had a fainter reaction (due to cross-reactivity).
DO you see what I am saying here? There is absolutely no doubt that Lion's LR3 IGF-1 contains BSA and a LOT of it. 100% sure; stake my life and reputation on it!
There is some slight chance that there is ALSO a little HSA in it. Since we couldn't do the diluted run, we can't rule that out completely. I really doubt that is the case though. Ultimately though, it doesn't matter. The fact that there are high levels of BSA in it make this product dangerous to life and health. Any small amounts of HSA present won't change that.
Lion has lied to you and everyone else about nearly every aspect of this product.
LION SAYS: receptor grade stuff incorporates the whole sequence for IGF-1 with a 13 amino acid side chain to prolong biological half life. It was developed for human use and is the cleanest, best stuff to use for maximum results. (paraphrasing of course)
THE TRUTH: Receptor grade IGF-1 is not the whole intact protein and was never intended to use in live animals or humans. It does not possess the same biological activity of native IGF-1 and in fact, may actually hinder one from utilizing their own IGF-1. It can only be detrimental to growth; not helpful to it.
LION SAYS: The diluent he sells is the best one because it contains the binding protein neccesary for stabilizing the IGF-1 (thus the BP in the name). All other diluents are inferior.
THE TRUTH: The diluent he sells contains no protein of any kind. It is just salt water. The BP in the name simply refers to the grade of saline. Fisher Scientific sells no less than five different grades including USP and BP grades. BP refers to British Pharmacopeia (USP stands for US Pharmacopeia).
LION SAYS: The product is completely human grade and contains no BSA (as per PM's to Skyefire and myself on Superior Muscle).
THE TRUTH: The product, in fact, contains an enormous amount of BSA and even if it didn't, it could not be considered "human grade" since receptor grade proteins were not developed for use in ANY living thing, little lone humans.
Something to keep in mind from one of my earlier posts on this subject. These growth mediums stabilized with BSA contain many more bovine proteins and hormones than just BSA. What they do is just add plain old bovine serum (or fetal bovine calf serum). The serum contains all the proteins and hormones you would expect to find in cow blood. Albumin is just the major protein fraction found in blood so there is more of that than any of the others. There is still going to be things like bovine insulin, bovine growth hormone, etc. A similar danger of immune response exists for all of these bovine proteins; not just the BSA.
Remember, mice injected with HSA DIE WITHIN 4 OR 5 DAYS due to massive hemmorages in their kidneys. This is due to an immune response where they begin forming antibodies to mouse serum albumin. This causes their blood to start clotting all over the place. Apparently, this first happens in their kidneys and the blood clots rupture blood vessels and they bleed to death before further immune response can clot the rest of their blood up. Looks pretty damn painful actually.....
__________________
for those of you that use lion products, here's another warning to stay away.
posted by liftsiron:
Lion's IGF-1 http://www.premiermuscle.com/forum/showthread.php?t=5957
It was tested by a ph.d chemist at an indepedant lab, here is what was found.
The test is conclusive. There is BSA in the IGF-1 and a buttload of it! The lighter signal for HSA may come from the cross-reactivity I mentioned (very likely).
We also tested it to see if it was
1. receptor grade
2. human IGF-1.
There was initially some ambiguity as to whether some of the IGF was porcine (pig) because of some slight cross reactivities. The diluted experiment clarified that. It is human and it is receptor grade. unfortunately, we ran out of sample before we could do the diluted run to determine if the HSA signal was just cross reactivity related or if there is actually a little HSA in there along with the gobs of BSA. I am 99% certain there is no HSA and here is why:
Pharmaceutical preps of IGF-1 do exist but they are the whole intact protein, not just the binding region (receptor grade). No receptor grade IGF or other receptor grade protein was ever developed for human use or even animal use. Receptor grade proteins are strictly used to determine binding constants and stuff like that in a petre dish. Since receptor grade was never intended for human use, it is produced in growth medium that contains BSA as a stabilizer (BSA is cheap). There would be no incentive to use more expensive HSA to stabilize something not intended for human use. When it was lyophylized (freeze dried), the BSA was included to stabilize the LR3 IGF-1. Without it, the IGF-1 would just fall apart in storage.
Lion does not make this stuff. There are only a handful of places that do make it (5 to be exact). He has to buy it from one of those companies. They all likely use BSA to stabilize their product.
This stuff isn't fit to inject into an animal, little lone a human being.
I will try to be more clear in my expanation here: Lion's stuff was applied to a plate with BSA antibodies in one column and HSA antibodies in another separate column. When Lion's stuff was applied to antibodies for BSA they lit up like Chernobyl! Definite positive for BSA. There was also a lighter reaction with the HSA antibodies. This was caused by our overloading the sample and causing a little cross reactivity. BSA and HSA are very similar proteins after all.
Just for argument's sake, let's say that it was HSA and no BSA was present. What we would have seen is the opposite. The HSA antibodies would have lit up brightly and the BSA antibodies would have had a fainter reaction (due to cross-reactivity).
DO you see what I am saying here? There is absolutely no doubt that Lion's LR3 IGF-1 contains BSA and a LOT of it. 100% sure; stake my life and reputation on it!
There is some slight chance that there is ALSO a little HSA in it. Since we couldn't do the diluted run, we can't rule that out completely. I really doubt that is the case though. Ultimately though, it doesn't matter. The fact that there are high levels of BSA in it make this product dangerous to life and health. Any small amounts of HSA present won't change that.
Lion has lied to you and everyone else about nearly every aspect of this product.
LION SAYS: receptor grade stuff incorporates the whole sequence for IGF-1 with a 13 amino acid side chain to prolong biological half life. It was developed for human use and is the cleanest, best stuff to use for maximum results. (paraphrasing of course)
THE TRUTH: Receptor grade IGF-1 is not the whole intact protein and was never intended to use in live animals or humans. It does not possess the same biological activity of native IGF-1 and in fact, may actually hinder one from utilizing their own IGF-1. It can only be detrimental to growth; not helpful to it.
LION SAYS: The diluent he sells is the best one because it contains the binding protein neccesary for stabilizing the IGF-1 (thus the BP in the name). All other diluents are inferior.
THE TRUTH: The diluent he sells contains no protein of any kind. It is just salt water. The BP in the name simply refers to the grade of saline. Fisher Scientific sells no less than five different grades including USP and BP grades. BP refers to British Pharmacopeia (USP stands for US Pharmacopeia).
LION SAYS: The product is completely human grade and contains no BSA (as per PM's to Skyefire and myself on Superior Muscle).
THE TRUTH: The product, in fact, contains an enormous amount of BSA and even if it didn't, it could not be considered "human grade" since receptor grade proteins were not developed for use in ANY living thing, little lone humans.
Something to keep in mind from one of my earlier posts on this subject. These growth mediums stabilized with BSA contain many more bovine proteins and hormones than just BSA. What they do is just add plain old bovine serum (or fetal bovine calf serum). The serum contains all the proteins and hormones you would expect to find in cow blood. Albumin is just the major protein fraction found in blood so there is more of that than any of the others. There is still going to be things like bovine insulin, bovine growth hormone, etc. A similar danger of immune response exists for all of these bovine proteins; not just the BSA.
Remember, mice injected with HSA DIE WITHIN 4 OR 5 DAYS due to massive hemmorages in their kidneys. This is due to an immune response where they begin forming antibodies to mouse serum albumin. This causes their blood to start clotting all over the place. Apparently, this first happens in their kidneys and the blood clots rupture blood vessels and they bleed to death before further immune response can clot the rest of their blood up. Looks pretty damn painful actually.....
__________________
